BLIND SEPARATION OF NEURONAL ACTIVITIES FROM OPTICAL IMAGING DATA

Susumu Takahashi, Yuichiro Anzai, Yuki Kobayashi, Takeshi Aihara, Minoru Tsukada
ftsusumu,anzaig@ayu.ics.keio.ac.jp, fk­yuki,aihara,tsukadag@eng.tamagawa.ac.jp

We applied Independent Component Analysis(ICA) to data of neuronal activities in the brain acquired by op­ tical imaging method. We constructed a model of noise and membrane potential mixed sources, and using suit­ able parameters detected from its simulation based on ICA, succeeded in estimating activities of neurons em­ bedded in optical imaging data from hippocampal s­ lices. Comparing our results with those obtained by the averaging set of data popular in the application of optical imaging method, it was empirically that our method could extract neuronal activities in the brain by one­shot data without averaging, which may pro­ vides a more precise method for the analysis of the complex mechanisms of the brain.